We describe in this edition a single, convenient system for both cloning and site-directed mutagenesis including deletions, base substitutions and base insertions. In-Fusion HD * Cloning Plus is ...

Reverse transcriptase-PCR (RT-PCR)--a method for assessing gene expression, detecting low copy number mRNA transcripts, and generating complementary DNAs (cDNAs) for cloning--converts an RNA template ...

A team of scientists report a new method for identifying any genome sequence located next to a known sequence. Sonication Inverse PCR (SIP) can be used to characterize any DNA sequence (near a known ...

Mullis used the basis of Sanger's DNA sequencing as a starting point for his new technique. He realized that the repeated use of DNA polymerase triggered a chain reaction resulting in a specific DNA ...

PCR is a technique used to amplify target DNA in a sample. It’s a well-known method that has undergone numerous modifications to enhance its capabilities. This year, it’s turning 40 years old. PCR has ...

Polymerase chain reaction (PCR) is a gene amplification technique that amplifies a single or a few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of ...